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Contrary to oligo(dT) primers, these primers aren't template precise and therefore are well suited for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis applying reverse transcriptase with mRNA templates.

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In difference to random primers, oligo (dT) primers usually are not ideal for degraded samples but are fantastic for setting up cDNA libraries and encouraged for use with reverse transcriptases (RTs) as Superscript IV RT.

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